SSRome is a fully functional, comprehensive, integrated database and pipelines for microsatellites mining, classification, comparative analysis and markers development across all organisms. In SSRome database, 158,041,204 microsatellite DNA motifs were identified across all annotated genomes of Plants, Metazoa, Archaea, Bacteria, Viruses, Fungi and Protozoa. In addition to all annotated Mitochondrial and Chloroplast genomes and all available NCBI ESTs till March 2018, represent collectively 6533 living organisms. This huge number of identified SSRs motifs were detected based on analysis of 300,159,904,537 bases of DNA. Moreover, a total of 45,109,613 SSR primer pairs were designed and classified in two main categories (genic or non-genic primers) according to their localization. All stored datasets can be downloaded freely in very convenient way. In addition to the SSRome database, SSRome provide three user-friendly in-house pipelines, can help scientists\users to identify, classify and compare microsatellite motifs in both genomic and transcriptomic-wide levels. This developed SSRome pipelines comes into three functional modules, to cover different types of SSRs mining analysis. The modules including ‘SSRome Genomic Pipeline', 'SSRome Transcriptomic Pipeline' and 'SSRome Comparative Analysis Pipeline'.