real-time quantitative PCR Primer Database
Names
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real-time quantitative PCR Primer Database
metasource: Fairsharing.org
version: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
qPrimerDB
metasource: Fairsharing.org
version: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657
Names
More detailed information about this field from each metasource.
real-time quantitative PCR Primer Database
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
qPrimerDB
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657
Names
More detailed information about this field from each metasource.
real-time quantitative PCR Primer Database
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
qPrimerDB
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657
The qPrimerDB database stores information on qPCR primers. Accessible through a web front-end providing gene-specific and pre-computed primer pairs across 147 different organisms, including human, mouse, zebrafish, yeast, thale cress, rice, and maize. The qPrimerDB provides an interactive and information-rich web graphical interface to display search and BLAST results as table-based descriptions and associated links. In this database, we provide 3,331,426 of the best primer pairs for each gene based on primer pair coverage (PPC), as well as 47,726,569 alternative gene-specific primer pairs, which can be conveniently batch downloaded. We validated the specificity and efficiency of the qPCR primer pairs for 66 randomly selected genes in six different organisms through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis.
Description
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The qPrimerDB database stores information on qPCR primers. Accessible through a web front-end providing gene-specific and pre-computed primer pairs across 147 different organisms, including human, mouse, zebrafish, yeast, thale cress, rice, and maize. The qPrimerDB provides an interactive and information-rich web graphical interface to display search and BLAST results as table-based descriptions and associated links. In this database, we provide 3,331,426 of the best primer pairs for each gene based on primer pair coverage (PPC), as well as 47,726,569 alternative gene-specific primer pairs, which can be conveniently batch downloaded. We validated the specificity and efficiency of the qPCR primer pairs for 66 randomly selected genes in six different organisms through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis.
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerDB database, founded on an automatic gene-specific qPCR primer design and thermodynamics-based validation workflow. The qPrimerDB database is the most comprehensive qPCR primer database available to date, with a web front-end providing gene-specific and pre-computed primer pairs across 147 important organisms, including human, mouse, zebrafish, yeast, thale cress, rice and maize. In this database, we provide 3331426 of the best primer pairs for each gene, based on primer pair coverage, as well as 47760359 alternative gene-specific primer pairs, which can be conveniently batch downloaded. The specificity and efficiency was validated for qPCR primer pairs for 66 randomly selected genes, in six different organisms, through qPCR assays and gel electrophoresis. The qPrimerDB database represents a valuable, timesaving resource for gene expression analysis. This resource, which will be routinely updated, is publically accessible at http://biodb.swu.edu.cn/qprimerdb.
metasource: Nucleic Acid Research database catalogueversion: extracted_at: 2022-11-04T11:16:25.468657
Webpage:
http://biodb.swu.edu.cn/qprimerdb/Webpage
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http://biodb.swu.edu.cn/qprimerdb/
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
http://biodb.swu.edu.cn/qprimerdb
metasource: Nucleic Acid Research database catalogueversion: extracted_at: 2022-11-04T11:16:25.468657
Publications:
Publications
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http://dx.doi.org/10.1093/nar/gkx725
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657
Publications
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http://dx.doi.org/10.1093/nar/gkx725
metasource: Nucleic Acid Research database catalogueversion: extracted_at: 2022-11-04T11:16:25.468657
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polymerase chain reaction primers
metasource: Fairsharing.org
version: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
Tags
More detailed information about this field from each metasource.
polymerase chain reaction primers
metasource: Fairsharing.orgversion: FAIRsharing.org: qPrimerDB; real-time quantitative PCR Primer Database; DOI: https://doi.org/10.25504/FAIRsharing.qy4b7s; Last edited: Jan. 8, 2019, 1:25 p.m.; Last accessed: Jan 03 2022 7:08 p.m.
polymerase chain reaction primers
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