Minimum Information Required for A Glycomics Experiment - Mass Spectrometric Analysis

Other names: MIRAGE MS

MIRAGE (Minimum Information Required for A Glycomics Experiment) was created to improve the quality of glycomics data in the scientific literature. Researchers seeking to understand the biochemical structure–function relationships of carbohydrates require detailed descriptions of the assay conditions and the experimental results. Currently, these data are insufficiently reported in the literature. A basic description on the sample preparation workflow is required. In contrast to proteomics, different types of glycoconjugates require partially different release approaches, which in turn can have direct influence on the following conditions/parameters: released glycans or still attached to protein/lipid; type of glycan (N-glycan, O-glycan, proteoglycan fragment); and sample pre-treatment prior MS-Analyses (non, reduced, permethylated, endo/exoglycosidase digested, fluorescent label, online/offline LC-separation).


Name: Creative Commons Attribution 4.0 International (CC BY 4.0)



mass spectrometry assay mass spectrometry spectra

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